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Combinatorial
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Materials Needed
Teacher Preparation:
General information
The timeline for procedural sequence has some potential slow-down points. Putting the plates in a refrigerator will allow you some flex time for all those pop up schedule breakers like Pep Rallys. Here is an overview.
The specific details can be found in Wolkenberg Lab: Teacher Preparation
Don't forget: go to images.htm for the pictures.
1 - 5 days before lab day: Prepare/Obtain sterile LB (Luria-Bertani) media for liquid culture
Prepare/Obtain and pour 20 ~30 mL sterile LB agar plates for solid culture (this can be done by the students themselves)
1 day before lab day: Prepare an "overnight culture" of E. coli TG1
Prepare solutions for chemistry. Store the solutions at room temperature until the lab day.
lab day: Prepare 1/100 dilutions of "overnight culture"
Prepare solutions for chemistry
Here is an example of the plate set-up:
Materials Needed
Wolkenberg Lab:
Each student group will need the following materials:
6 - 1.5 mL Eppendorf tubes
1 - tube rack
7 - transfer pipets
1 - thin-tipped marker (for labeling)
3 - sterile LB agar plates
1 - glass pasteur pipet
1 - 1/100 dilution of E. coli TG1 overnight culture
