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Combinatorial
ChemistryFit ItView ItPrep ItDo ItAssess ItLink ItView It
Abstract
In this laboratory you will identify a "pharmaceutical" that kills bacteria. The technique is combinatorial in nature, meaning that multiple chemical compounds will be prepared (mixed) and screened for antibiotic activity. This is the significant concept of this laboratory.
In this experiment, you will produce mixtures (libraries) of compounds based on an A-B model to be discussed. You will simultaneously generate libraries, test them for antibiotic activity, and deconvolute (separate) them to discover which individual compound has antibiotic properties.
You will use the cup agar diffusion method to screen the mixtures for antibiotic activity.
The image on the left is pulling the "cup" from the agar. On the right is a "cup" that has been lifted out of the agar.
All images including the "School Solution" plate are found in the "Image" Folder. This message will be seen throughout the sequence and will always point you to the image folder.
Content information important for the teacher
This laboratory is the product of Scott Wolkenberg from the Scripps Research Institute and can I have created links to seperated sections of the lab and as an MSWord and in HTML format. Here are the links in both versions of the complete Wolkenberg Lab: Wokenberg MSWord and: Wolkenberg Lab HTML
This experiment and instruction manual are the product of the efforts of a dedicated group of graduate students (PhD Candidates) at The Scripps Research Institute. If you require any help or have any questions regarding the preparation or execution of this experimental exercise, please contact us, start with Scott. It is our hope that you will share this with your students and that they will benefit from it. If a technical detail is preventing you from performing the experiment, we will do anything we can to address it.
Scott Wolkenberg
BCC483
The Scripps Research Institute
10550 N. Torrey Pines Rd.
La Jolla, CA 92037
858-784-7528
scottw@scripps.edu
This sections is an extract from the lab written in "Teacher" and is meant to be copied. Included in this document are detailed instructions for conducting and preparing or obtaining all the materials needed for the experiment. A list of supplies is attached.
The results of the experiment are seen through the observation of inhibition of growth, or no inhibition of growth in the six cup tests for antibiotic activity of mixtures. Inhibition of growth is observed when a clear area of approximately 1.5-2 cm circle of no growth on the agar plate is observed around the agar cup.Attached to this material is a data analysis sheet which has been completed to identify the antibiotic compound. In other words, the answer. Intrduction in MSWord and HTML
Type of Lab
This lab demonstrates cutting edge schema which is scientifically pertinent to the biotechnology industry.
The lab is directed inquiry based, constructivist learning activity. Significant biochemistry is good to have relative to compound formation and activity.
Students are asked to select a solution which exhibits antibacterial activity.
Instructional Strategies Used
Lecture, discussion, group (2-4)/cooperative learning, independent research, guided practice based on previously learned laboratory procedures (solution chemistry, micro pipetting etc.)
